Optional programs and accessories – Microsampling

Small volumes of very precious samples have always been a challenge for CD measurement. Conventional micro-cells typically have volumes as low as about 60 μL, but a new breakthrough allows CD measurements for samples with volumes as small as 2 μL.

Micro sampling disk

The micro sampling disk is designed to measure samples as small as 2 μL. High-quality quartz windows with a special surface treatment utilize spacers of 1.0 and 0.2 mm for volumes of 10 and 2 μL, respectively. The sample is applied using a micropipette, and cleaning is quick and easy.

CD Spectra of DNA derived from bovine thymus

CD Spectra of hemoglobin

Comparison of secondary structural analysis

Helix (%) β-Sheet (%) Turn (%) Random (%)
Hemoglobin X-ray 78.0 0.0 6.3 15.7
Micro sampling disk 73.8 0.0 10.9 15.3
Cylindrical cell 72.2 0.0 12.0 15.8

Capillary cell jacket

For thermal ramping studies of precious samples we also offer the solution by the capillary cell jacket, which allows use of samples as small as 5 μL to be placed in the Peltier cell holder. Melting curves are easily measured, including calculation of Tm, ΔS and ΔH.

Tm measurement of Hemoglobin
(Wavelength: 222 nm, Temperature: 20 °C to 90 °C )


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