Small volumes of very precious samples have always been a challenge for CD measurement. Conventional micro-cells typically have volumes as low as about 60 μL, but a new breakthrough allows CD measurements for samples with volumes as small as 2 μL.
Micro sampling disk
The micro sampling disk is designed to measure samples as small as 2 μL. High-quality quartz windows with a special surface treatment utilize spacers of 1.0 and 0.2 mm for volumes of 10 and 2 μL, respectively. The sample is applied using a micropipette, and cleaning is quick and easy.
CD Spectra of DNA derived from bovine thymus
CD Spectra of hemoglobin
Comparison of secondary structural analysis
| Helix (%) | β-Sheet (%) | Turn (%) | Random (%) | ||
| Hemoglobin | X-ray | 78.0 | 0.0 | 6.3 | 15.7 |
| Micro sampling disk | 73.8 | 0.0 | 10.9 | 15.3 | |
| Cylindrical cell | 72.2 | 0.0 | 12.0 | 15.8 |
Capillary cell jacket
For thermal ramping studies of precious samples we also offer the solution by the capillary cell jacket, which allows use of samples as small as 5 μL to be placed in the Peltier cell holder. Melting curves are easily measured, including calculation of Tm, ΔS and ΔH.
Tm measurement of Hemoglobin
(Wavelength: 222 nm, Temperature: 20 °C to 90 °C )