Using the CD Spectrometer as a chiral HPLC detector of CD
HPLC is an effective method for quantitative detection of trace amounts of compounds and for mixture separation/ purification. HPLC is also used for analysis of medicinal products and in food science. In these fields, samples often have optical activity allowing the analysis of enantiomeric excess and the separation/purification of the enantiomers. JASCO has been a pioneer in the development of chiral HPLC detection and offers an HPLC flow-cell for J-1000 systems enabling acquisition of high-quality chromatographic data during chiral separations.
LCCD-420 Flow cell attachment for LC-CD
The LCCD-420 enables the J-1000 to operate as an HPLC CD detector, and the chromatographic signal can be output into any commercially available CDS (chromatography data system).
* JWAOM-514 Analog output function module program is required.
Application
HPLC-CD of warfarin
Warfarin was dissolved using a mixture of pH 2.0 aqueous phosphoric acid and acetonitrile. CD measurement was performed using a J-1500 outfitted with the LCCD-420 cell and connected to a conventional HPLC system.
CD spectra of Warfarin
Absorption spectra of Warfarin
CD chromatograms of Warfarin
Absorption chromatograms of Warfarin