Simultaneous quantitative analysis of multi-component in soybean

December 1, 2021


Near-IR spectroscopy is widely used for quantitative analysis of components in food product. Its method has the feature that it is possible to perform non-destructive measurement without any special sample preparation. Therefore, not depending on the status of sample (such as liquid, paste, solid or particle), just putting it on the sampling plate or cell in the analytical instrument can perform the measurement easily. In addition, since the multivariate analysis can provide the quantitative information of mult-component from one spectrum simultaneously without splitting the spectrum into several spectra, applying its technique enables to get a lot of information rapidly and easily.

This article shows the simultaneous quantitative analysis result of each component in commercial food product by a series of near-IR measurement.


FT/IR-4600 FTIR spectrometer


Soybeans were measured without destroying them by using Fourier-Transform near-IR spectrometer (FT-NIR). Figure 1 shows the near-IR spectra of each soybean. Although these spectra are similar, there are slight difference among them.

Figure 1. Near-IR spectra of each soybean

Next, PLS calibration models of each component were created by using measured near-IR spectra. Quantitative information of each component which is measured by wet chemistry method is used for the true value of PLS calibration model. Figure 2 shows the calibration models which were created simultaneously by using the measured value of other components (water, carbohydrate and fat) as well as protein. These models of each component were created with high correlation between the true value and the predicted value, and it means that this technique can quantify the multi-components

Figure 2. PLS calibration models

FT-NIR system has the following advantage comparing to the other spectroscopy system, and can be used for quantifying the multi-components in food product simultaneously.

  • Excellent wavenumber accuracy
  • Bright and high throughput
  • Continuous measurement and monitoring available
  • Simultaneous measurement in entire region

JASCO can suggest the suitable solution in accordance to the user’s demands.

About the Author

Spectroscopy Group