Fig. 7 Optical configuration of spectrofluorometer
In a spectrofluorometer, a xenon lamp is usually used. It emits light by discharge of xenon gas and has a wide energy distribution from UV to infrared. However, the intensity decreases in the UV region of 300 nm or less, but a bright line exists near 450 nm.
Fluorescence measurement cell
Fig. 8 Four-sided transparent cell (left) and triangular cell (right)