Fluorescence spectroscopy is routinely used for studying structural changes in conjugated systems, aromatic molecules, and rigid, planar compounds due to alterations in temperature, pH, ionic strength, solvent, and ligands. A single fluorophore can generate thousands of detectable photons that can be repeatedly excited and detected, making fluorescence spectroscopy is a highly sensitive technique.

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Table of contents

Principle

• What is fluorescence?
• Absorption and emission
• Excitation spectrum and fluorescence spectrum
• Advantages of fluorescence spectroscopy
• Solutions by fluorescence spectroscopy

Instrument Introduction

• FP-8050 Series Spectrofluorometer

LIFE SCIENCE APPLICATIONS

• Fluorescence measurement of heat-denatured protein
• Monitoring of protein behavior by using FRET
• Sensitivity evaluation of biosensor by using FRET
• Fluorescence depolarization measurement
• Enzyme kinetics probed by fluorescence spectroscopy
• Measurement of trypsin activity using fluorescence peptide substrate
• Reaction process monitoring by fluorescence stopped-flow system
• Quantitative analysis of λDNA using One drop accessory

MATERIAL SCIENCE APPLICATIONS

• Fluorescence quantum yield measurement
• Internal quantum efficiency measurement of phosphor powders
• Phosphorescence measurement
• Remote fluorescence measurement using optical fiber
• Evaluation of up-conversion phosphor

ENVIRONMENTAL SCIENCE APPLICATIONS

• Evaluation of environmental water using PARAFAC
• High-Speed Excitation Emission Matrix Measurement of Olive Oil
• Petroleum product assessment by counmarin analysis