Application eBook – Fluorescence Spectroscopy

August 27, 2021

Fluorescence spectroscopy is routinely used for studying structural changes in conjugated systems, aromatic molecules, and rigid, planar compounds due to alterations in temperature, pH, ionic strength, solvent, and ligands. A single fluorophore can generate thousands of detectable photons that can be repeatedly excited and detected, making fluorescence spectroscopy is a highly sensitive technique.

Table of contents

Principle

  • What is fluorescence?
  • Absorption and emission
  • Excitation spectrum and fluorescence spectrum
  • Advantages of fluorescence spectroscopy
  • Solutions by fluorescence spectroscopy

Instrument Introduction

  • FP-8050 Series Spectrofluorometer

LIFE SCIENCE APPLICATIONS

  • Fluorescence measurement of heat-denatured protein
  • Monitoring of protein behavior by using FRET
  • Sensitivity evaluation of biosensor by using FRET
  • Fluorescence depolarization measurement
  • Enzyme kinetics probed by fluorescence spectroscopy
  • Measurement of trypsin activity using fluorescence peptide substrate
  • Reaction process monitoring by fluorescence stopped-flow system
  • Quantitative analysis of ╬╗DNA using One drop accessory

MATERIAL SCIENCE APPLICATIONS

  • Fluorescence quantum yield measurement
  • Internal quantum efficiency measurement of phosphor powders
  • Phosphorescence measurement
  • Remote fluorescence measurement using optical fiber
  • Evaluation of up-conversion phosphor

ENVIRONMENTAL SCIENCE APPLICATIONS

  • Evaluation of environmental water using PARAFAC
  • High-Speed Excitation Emission Matrix Measurement of Olive Oil
  • Petroleum product assessment by counmarin analysis