Micro Sample Analysis using ATR PRO 4X VIEW

February 21, 2023


The use of ATR is an effective method for fast and efficient IR measurement. When using ATR, it is necessary to correctly set and press the sample at the center of the prism to obtain good quality data, especially when measuring a micro sample which may be smaller than the prism.


JASCO’s latest accessory, the ATR PRO 4X VIEW has an observation function to locate the ideal measurement position, while displaying the sample area on the front monitor or PC.

In this application note we tested several cosmetic eyelashes. There are many different types of false eyelashes for cosmetic purpose, which are claimed to be made from human hair, animal hair, or natural fibers according to the material labeling. However, false eyelashes are often labeled as from animal sources when in fact they are made from synthetic fabric. The analytical evaluation between human eyelashes and false eyelashes claimed as human hair is made here using the ATR PRO 4X VIEW.


Measurement Method
Set the human eyelash and the false eyelash claimed as human hair commercially on the center of the prism using the monitor. Measurement conditions are shown below.

Instruments FT/IR-4X
Resolution 4 cm -1
Method ATR
Detector DLATGS
Accumulation 50 times
Accessory ATR PRO 4X VIEW
Prism PKS-D1V


130-AT-0244, Micro sample, Material analysis, ATR


Figure 2 shows the sample images and the IR spectra of a human eyelash and a false eyelash. As shown in the images, the samples were set at the center of the prism. Peaks derived from Amide I, II are detected around 1640 cm–1 and 1550 cm–1 in the human eyelash IR spectrum, which means that main component of human eyelash is protein.

Figure 2. IR spectra of human eyelash (left) and false eyelash (right)

The spectrum of the false eyelash IR is different from human’s, and indicates the existence of a synthetic fiber. The main component was identified as polybutylene terephthalate by database searching.

Figure 3. IR spectra of polybutylene terephthalate standard (top) and false eyelash (bottom)


The ATR PRO 4X VIEW is a fast and effective tool for measuring micro samples, in this case the main component of the false eyelash was identified as polyester.

In general, the application of non-destructive analytical techniques for micro samples is very important for foreign material analysis, criminal investigation and false labeling.

This note shows that ATR PRO 4X VIEW is highly effective for non-destructive micro sample analysis in a variety of application fields.

Because the ATR PRO 4X VIEW provides not only analytical information but also spatial information like sample view and size.


About the Author

Dr. Carlos Morillo received his Post Doc at Advanced Industrial Science & Technology in Fukuoka and was a Research Scientist at Kyushu University in Japan where he lived for several years. Carlos received his Doctor of Engineering from Kyushu University and his Masters and BS from Simon Bolivar University in Caracas Venezuela. He is an Applications Scientist at JASCO.

About the Author

Spectroscopy Group