Introduction

The use of commercial sweeteners in the manufacture of food and drinks is strictly regulated by agencies such as the FDA. This application note demonstrates a robust method for the analysis of saccharin (sodium salt), described in a Food Additive Test Method posted in an annotation of the Food Hygiene Law.

LC-4000 HPLC system

Experimental

Chromatographic conditions
Column: CrestPak C18S (4.6 mmI.D. x 150 mmL, 5 µm)
Eluent: 0.05 mol/L Phosphate buffer (pH 6.9)/Methanol (90/10)
Flow Rate: 1.0 mL/min
Column Temp: 40ºC
Wavelength: 230 nm
Injection Volume: 10 µL
Standard Sample: Saccharin (sodium salt) 0.5 µg/mL in water

Structural formula of saccharin (sodium salt) is shown in Figure 1.

Figure 1. Structural Formula of Saccharin (Sodium salt)

Keywords

Food additive, Sweetener, Saccharin sodium, C18 column, UV detection HPLC

Results

A chromatogram of a saccharin standard is shown in Figure 2.

Figure 2. Chromatogram of a Standard Sample of Saccharin