The use of commercial sweeteners in the manufacture of food and drinks is strictly regulated by agencies such as the FDA. This application note demonstrates a robust method for the analysis of saccharin (sodium salt), described in a Food Additive Test Method posted in an annotation of the Food Hygiene Law.
LC-4000 HPLC system
Column: CrestPak C18S (4.6 mmI.D. x 150 mmL, 5 µm)
Eluent: 0.05 mol/L Phosphate buffer (pH 6.9)/Methanol (90/10)
Flow Rate: 1.0 mL/min
Column Temp: 40ºC
Wavelength: 230 nm
Injection Volume: 10 µL
Standard Sample: Saccharin (sodium salt) 0.5 µg/mL in water
Structural formula of saccharin (sodium salt) is shown in Figure 1.
Figure 1. Structural Formula of Saccharin (Sodium salt)
Food additive, Sweetener, Saccharin sodium, C18 column, UV detection HPLC
A chromatogram of a saccharin standard is shown in Figure 2.
Figure 2. Chromatogram of a Standard Sample of Saccharin