Investigation of Chiral Separation Conditions of Flavanone by Supercritical Fluid Chromatography Methods Scouting System

January 25, 2021


In the synthesis of medicinal drugs and agrichemical fields, synthetic compounds with optical activity are continuing to gain more attentions. These compounds can show the same physical chemical properties, but show different biological activity.

It has been reported that using only one enantiomer of the optical isomer can enhance the medicinal effects and reduce the side effects when the biological activity varies between the enantiomers.

Our Methods Scouting System makes it simple for users to search and select the appropriate measurement conditions using various solvents and columns for both chiral and achiral separations.

Supercritical fluid chromatography (SFC) is well known for quick separations, easy solvent replacement, easy sample treatment after preparation, decreasing solvent cost over HPLC and simple scale-up from analytical to preparative.

In this article, method scouting of flavanone with three modifiers and six columns is carried out by using SFC system and Method Scouting Program.

SFC method scouting system


Experimental Condition
Column:   CHIRALPAK IA, IB, IC, ID, IE and IF/SFC (i-CHIRAL 6), (4.6 mmI.D. x 150 mmL, 5 µm)
Eluent:   CO2/modifier (75/25)
Modifier:  1) methanol, 2) acetonitrile/ethanol (80/20), 3) methyl tert-butyl ether(MTBE)/ethanol (80/20)
Flow rate:   3.0 mL/min
Column temp.:   40 ̊C
Wavelength:   UV: 210 nm, CD: 310 nm
Back pressure.:  15 MPa
Injection volume:   5 µL
Standard:   1.0 mg/mL flavanone in methanol



Schematic diagram


Flavanone, SFC, Method Scouting Program, i-CHIRAL 6, Chiral separation


Figure 1 and 2 show the results of Method Scouting by UV (PDA) detector and CD detector. This view provides easy viewing of all screening conditions and quick determination of the best for further optimization if needed.

Figure 1. Result of Method Scouting (UV detector)

Figure 2. Result of Method Scouting (CD detector)

Table 1 shows the degree in separation of flavanone. As shown in the table, methanol and CHIRALPAK IF is suited to this measurement. Rapid separation can be achieved in 3 minutes using CHIRALPAK IA or ID.

Table 1 Degree in separation

Methanol 5.46 N.S. N.S. 7.63 2.44 10.1
Acetonitrile/Ethanol (80/20) 3.46 N.S. N.S. 4.10 2.17 6.80
MTBE/Ethanol (80/20) 4.12 N.S. N.S. 6.05 2.70 9.13

N.S.: Not Separated

About the Author

Chromatography Group