Analysis of RS-Ibuprofen with circular dichroism (CD) detector

December 14, 2020


Ibuprofen is a well known non-steroidal anti-inflammatory drug and widely used for pain relief and fever reduction. Only the S enantiomer of ibuprofen has active antipyretic properties, but within the human body an enzymatic reaction can readily convert the R enantiomer into the S enantiomer.

This article shows the separation result of RS-ibuprofen in the standard sample and commercial cold tablet by using a dedicated HPLC circular dichroism detector.

LC-4000 HPLC system


Chromatographic conditions
Column: CHIRALCEL OD (4.6mm I.D. x 250mmL)
Eluent: n-Hexane/ IPA/ TFA (100/1/0.1)
Flow rate: 1.0ml/min
Column temperature: 25 degree celsius
Sample: RS-Ibuprofen (0.1mg/mL), Cold tablet
Injection volume: 10uL
Detection: Circular dichroism
Wavelength: 235nm
Scale: 1mV =1mdeg
Response: Standard
Polarity: +


Ibuprofen, STD, Cold tablet, CHIRALCEL OD, Circular dichroism detector, 5 .Chiral separation


Figure 1 shows the RS-ibuprofen optically resolved CD chromatogram and the simultaneously detected UV chromatogram from the Circular dichroism detector.

Figure 1. CD and UV chromatograms of RS-ibuprofen

Figure 2 shows the CD and UV chromatograms of a sample extracted from a commercial cold medication.

Figure 2. CD and UV chromatograms of cold medication

About the Author

Chromatography Group