Introduction
Flurbiprofen is a non-steroidal anti-inflammatory drug and used as a topical medical patch or an internal medicine. The S enantiomer of flurbiprofen, accounts for the majority of the pharmacological activity.
The Circular Dichroism detector is well known for its high accuracy and selectivity for chiral analysis. Both CD and UV signal are measured simultaneously and spectral scanning can be used to optimize the wavelength of interest, which is important in the measurement of chiral compounds. Not only can the CD detector help differentiate between two enantiomers, but it can also distinguish between achiral impurities and enantiomers of interest.
LC-4000 HPLC system
Experimental
Chromatographic conditions
Column: CHIRALPAK AD-RH (4.6 mmI.D. x 150 mmL, 5 µm)
Eluent A: 0.1% phosphoric acid aqueous solution
Eluent B: Acetonitrile
Composition: Eluent A/B (50/50)
Flow rate: 0.5 mL/min
Column temp.: 25ºC
Wavelength: 250 nm
Response: 1.5 sec
Scan speed: 10 nm/sec
Injection volume: 10 µL
Standard sample: 100 µg/mL racemic flurbiprofen in eluent A/B (50/50)
Flurbiprofen
Results
Figure 1 shows the chromatograms of the racemic flurbiprofen standard. Figure 2 shows CD and UV spectra (measured by spectral scanning).
Figure 1. Chromatogram of Flurbiprofen standard (Top: CD detection, Bottom: UV detection)
Figure 2. Spectral measurement (Top: CD, Bottom: UV)
