For high-precision CD spectrum measurement, [Wavelength Accuracy] and [Wavelength Repeatability] are important parameter to obtain precise spectrum. 253.652 nm emission line spectrum of mercury lamp is good reference to verify [Wavelength Accuracy] and [Wavelength Repeatability] of CD spectrometer.
This article shows the validation result of [Wavelength Accuracy] and [Wavelength Repeatability] of CD spectrometer by using mercury lamp.
J-1500 CD spectrometer
Using the mercury lamp which is installed to JASCO’s CD spectrometer as standard, 253.652 nm mercury lamp emission line spectrum was measured five times.
Photometric mode: DC
HT voltage: Manual (300 V)
Measurement range: 250-260 nm
Data acquisition interval: 0.025 nm
Response: 0.125 sec
Scan speed: 10 nm/min
Wavelength accuracy, Wavelength repeatability, Mercury lamp
Figure 1, 2 and Table 1 show the repeat measurement results of the 253.652 nm mercury lamp emission line spectrum. Five spectra show good agreement, so high [Wavelength Accuracy] and [Wavelength Repeatability] were verified.
Figure 1. Mercury lamp emission line spectra
Figure 2. Mercury lamp emission line spectra (right: Zoom view)
Table 1. Wavelength repeatability