Remote fluorescence measurement using optical fiber

April 9, 2021

Introduction

JASCO has various accessories for supporting the user’s measurement, and these accessories can be mounted to spectrofluorometer easily. One of JASCO’s accessories is optical fiber interface, which enables the fluorescence measurement by holding the probe close to or immersed in the sample. A spectrofluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure. This section shows the measurement example by using spectrofluorometer with optical fiber.

FP-8550 Spectrofluorometer

Experimental

Fluorescence measurements of fluorescent reflector (red/yellow) were performed by using spectrofluorometer, optical fiber and dark chamber. The spectral correction was also performed by using the reflected light of the calibrated WI lamp from standard white plate.

Figure 1. Overview of measurement system

 

Results

Figures 2 and figure 3 show the measurement results. The profiles of the spectra of each colored sample measured using both the optical fiber and film holder were consistent with each other.

Figure 2. Peak normalized fluorescence spectrum of the yellow fluorescent reflector, excitation wavelength: 310 nm

Figure 3. Peak normalized fluorescence spectrum of the red fluorescent reflector, excitation wavelength: 310 nm

Conclusion

This system is effective in the case such as the measurement of a sample which is larger than sample chamber and the measurement under the severe environment.

The solution examples by using optical fiber are shown as below.

In addition, JASCO can provide the useful optional software to convert the luminous/rendering color into numerical values and to evaluate them quantitatively. It has the calculation function of each color model which CIE (International Commission on Illumination) has published, and is also compliant with JIS regulation (color specification). Since it includes pass/fail evaluation function, it will be powerful tool to manage the luminous color of product.

[Luminous Color Analysis] program

About the Author

Spectroscopy Group