Introduction

Disulfiram is a drug used to treat chronic alcoholism by producing an acute sensitivity to alcohol. This article shows the utility of an X-Press PakC18S column (2.1mm I.D. x 50mmL.) packed with 2µm diameter packing material for the ultra-high speed separation of the above drug, and also shows the results to determine whether the performance of the column and chromatography separation exceed those of conventional HPLC.

LC-4000 UHPLC system

Experimental

Chromatographic conditions
Column = X-Press PakC18S (2.1mmI.D.x50mmL.)
Mobile phase = CH₃OH/H₂O(70/30)
Column temperature = 25 ºC
Flow rate = 0.6 mL/min
Detection= UV absorption (210 nm)
Injection volume = 1 µL

Results

Figure 1 shows the separation of a standard mixture of benzophenone (0.005mg/mL) and disulfiram (0.005mg/mL). UHPLC system provides an analysis time 7 times shorter than conventional HPLC while the resolution between the benzophenone and disulfiram was 5.4; the reproducibility of the peak ratio is 0.44%. These results well exceed those of conventional HPLC.

Figure 1. UHPLC chromatogram of a standard mixture of benzophenone and disulfiram (1 = benzophenone (0.005mg/mL), 2 = disulfiram (0.005mg/mL))