Paper published on Applied Spectroscopy

October 7, 2022

We are pleased to announce that new paper has been published on Applied Spectroscopy.

Performance Comparison of Spectral Distance Calculation Methods – Taiji Oyama, Satoko Suzuki, Yasuo Horiguchi, Ai Yamane, Kenichi Akao, Koushi Nagamori, Kouhei Tsumoto, 2022 (


Circular dichroism (CD) spectroscopy is a widely used technique for assessing the higher-order structure (HOS) of biopharmaceuticals, including antibody drugs. Since the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use established quality control guidelines, objective evaluation of spectral similarity has been required in order to assess structural comparability. Several spectral distance quantification methods and weighting functions to increase sensitivity have been proposed, but not many reports have compared their performance for CD spectra. We constructed comparison sets that combine actual spectra and simulated noise and performed a comprehensive performance evaluation of each spectral distance calculation method and weighting function under conditions that consider spectral noise and fluctuations from pipetting errors.

This algorithm has also been implemented in the qHOS program and can be used practically in spectra comparison and similarity test process.

You can learn more about qHOS on following sites.

Product information -qHOS-

qHOS – Higher order structure similarity evaluation software –

Poster session at PEGS BOSTON 2022

Orthogonal HOS Similarity Assessment of Biosimilar Using Multi Spectroscopic Technique and Statistical Calculation

About the Author

Spectroscopy Group