Features of P-4000 – Stable and Accurate Measurement –

Usage of sodium lamp

JASCO can offer the solution about optical rotation measurement by using sodium lamp ([α]D), which several pharmacopoeias recommend. LED and tungsten lamp are also available as option, and the range of selectable measurement wavelengths can be expanded.

<Correction function when using LED and tungsten lamp>
Compared to sodium and mercury lamps, measurement wavelength by using LED and tungsten lamp heavily depends on the performance of the selected optical filter (refer to the bottom figure).
P-4000 series has the optical rotation correction function, allowing the data measured with LED and tungsten lamp to be corrected using sodium lamp and standard quartz control plate.

ORD spectrum of pirarubicin, sodium lamp emission line and the transmission spectrum of optical filter

Sample temperature control/monitoring

Following useful functions to control/monitoring the sample temperature are available as standard.

  • Usage of Peltier element for accurate temperature control
  • Temperature sensor for direct sample temperature monitoring

<Importance of sample temperature>
Several pharmacopoeias specify sample temperature during measurement because the optical rotation is affected by sample temperature. Therefore, proper temperature control and monitoring are required.
P-4000 series has the auto measurement start function, allowing measurements to begin automatically once the set temperature is reached.

Sample temperature control Specification
Setting temperature range 15 to 40 °C
Temperature control accuracy ± 0.2 °C (when using holder sensor)
Temperature monitoring accuracy ± 0.1 °C

Durable sample compartment

Following features prevent instrument degradation caused by the acidic samples.

  • Temperature sensor coated with fluoresin
  • Sample compartment inner wall coating
  • Sample compartment window for inflow prevention of the acid gas into the inside of instrument
  • Acid gas exhaust function
  • Connection of exhaust duct (option)

Sample compartment window

Exhaust port

Instrument validation by using standard quartz control plate with ISO/IEC 17025 compliant

Validation which is described in several pharmacopoeias can be performed by using calibrated standard quartz control plate.

<ISO/IEC 17025 Accreditation>
JASCO* has received ISO/IEC 17025 accreditation to meet the calibration needs of measurement instrument.
Calibration certificates issued by JASCO include accreditation symbols (JAB and ILAC-MRA), recognized worldwide.
*JASCO calibration division

User-friendly validation and daily check operations

Validation program in P-4000 series verifies the instrument performance using the inspection items described in pharmacopoeias (JP/USP/EP). The operation following the dialog is simple, allowing easy execution of validation.

Test items JP USP EP Basic
Repeatability of zero optical rotation ✓
Repeatability of optical rotation ✓ ✓
Accuracy of optical rotation ✓ ✓ ✓ ✓
Temperature control ✓
Linearity of optical rotation ✓ ✓

<Supports of daily check>
Daily check program can monitor the instrument condition by checking the optical rotation of quartz control plate and the intensity of light source before/after daily measurements.
(Check items)

  • Optical rotation of calibrated quartz control plate
  • Intensity of light source (HT voltage)

Registration of quartz control plate for check

Only P-4200/4300 available